A
Specimen: Serum
Principle and method: Kinetic Colorimetric
Specimen Volume: 1 mL
Rejection: Grossly Hemolyzed Sample
Turnaround Time (TAT): Daily
Department: Clinical Biochemistrty
Specimen: Sodium Citrate Plasma
Specimen Stability: Sample stable for 4 hours at Room Temperature.
Principle and method: Coagulometry
Specimen Volume: 1 mL
Device: Sysmex CA-1500
Limitation: Test Result Noticeably increases by hemolysis.
Rejection: Grossly Hemolyzed Sample
Turnaround Time (TAT): Daily
Department: Hemostasis
Specimen: Sodium Citrate Plasma
Specimen Stability: 4 Hours at Room Temperature, Or 4 Weeks at -20 C°.
If the sample needs to be frozen, recentrifuge the withdrawn plasma and rapidly freeze the supernatant in a well-closed plastic container.
Principle and method: Coagulometry
Specimen Volume: 1 mL
Device: Sysmex CA-1500
Turnaround Time (TAT): Daily
Department: Hemostasis
Specimen: Heparin Plasma (Refrigerant)
Principle and method: High Performance Liquid Chromatography (HPLC)
Specimen Volume: 1 mL
Device: API 3200 LC/MS/MS System
Turnaround Time (TAT): (10 Days)
Department: Advanced Analytical Chemistry
Specimen: Serum , Pleural Fluid, CSF (All Samples Must be Refrigerant).
Sample Preparation: Pleural fluid should be collected in a sterile or heparinized tube. CSF Should be clear and collected in a sterile tube without anticoagulant.
Specimen Stability:
Serum stable at 2-4 C° for One week
Pleural Fluid stable for 2 Hours at Room Temperature, 2 days at 2-4 C°, 2 days at -20 C°.
CSF stable 24 hours at 25 C°, 7 days at 4 C° and 3 months at -20 C°.
Keep serum tightly stoppered.
Principle and method: Enzymatic Colorimetric
Specimen Volume: 1 mL
Device: Roche (cobas c 501)
Turnaround Time (TAT): Daily
Department: Clinical Biochemistry
Specimen: EDTA Plasma (Frozen)
Patient Preparation: For the 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.
Collection Instructions:
1. Morning (6 a.m.-10:30 a.m.) specimen is desirable.
2. Collect with a pre-chilled lavender top (EDTA) tube and transport to the laboratory on ice.
3. Centrifuge at refrigerated temperature within 2 hours and immediately separate plasma from cells.
4. Immediately freeze plasma.
Specimen Stability: Stable for 2 hours at 22 °C, 4 weeks at ‑20 °C (± 5° C). Freeze only once.
Specimen Volume: 1 mL
Turnaround Time (TAT): Daily
Limitation: ACTH results should always be evaluated together with simultaneously measured cortisol concentrations.
Aliases: Corticotropin
Principle and method: Electrochemiluminescence (ECLIA)
Device: Roche (Cobas e 601)
Department: Endocrinology
Specimen: EDTA Plasma (Frozen)
Patient Preparation: For the 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.
How the test is performed: 1 mg of dexamethasone is given to the patient at 11 p.m., and the blood is drawn at the morning for a ACTH measurement.
Collection Instructions:
1. Morning (6 a.m.-10:30 a.m.) specimen is desirable.
2. Collect with a pre-chilled lavender top (EDTA) tube and transport to the laboratory on ice.
3. Centrifuge at refrigerated temperature within 2 hours and immediately separate plasma from cells.
4. Immediately freeze plasma.
Specimen Stability: Stable for 2 hours at 22 °C, 4 weeks at ‑20 °C (± 5° C). Freeze only once.
Specimen Volume: 1 mL
Turnaround Time (TAT): Daily
Limitation: ACTH results should always be evaluated together with simultaneously measured cortisol concentrations.
Aliases: Dexamethasone Suppression Test, ACTH Post Dexa, ACTH Suppression test
Principle and method: Electrochemiluminescence (ECLIA)
Device: Roche (Cobas e 601)
Department: Endocrinology
Specimen Type: Wound, Bronchial Wash, Ear Swab, Urine, Vaginal Sec, Hair, Nasal Sec, Oral cavity, Prostatic Sec, Pus, Eyelid Swab, Fingernail, Secretion, Semen, Skin Legion, Skin Scraping, Sputum, Stool, Surgical Wound, Toenail, Throat Swab, Tongue Swab, Urethral Sec, Aerobic Blood.
Aliases: SGPT
Specimen Type: Serum
Specimen Volume: 1 mL
Specimen Stability: sample is stable for 3 days at 15‑25 °C, 7 days at 2‑8 °C, and more than 7 days at (-60)‑(-80) °C.
Special Instructions: Separate the serum or plasma from the clot or cells promptly.
Limitations: In patients with vitamin B6 deficiency, serum aminotransferase activity may be decreased.
Test Result Noticeably increases by hemolysis. Contamination with erythrocytes will elevate results, because the analyte level in erythrocytes is higher than in normal sera. The level of interference may be variable depending on the content of analyte in the lysed erythrocytes. Exceptions: Physiological plasma concentrations of Sulfasalazine and Sulfapyridine may lead to false results. Exception: Calcium dobesilate can cause artificially low ALT results at therapeutic concentrations.
Turnaround time (TAT): Daily
Principle and method: ElectroChemiLuminescence (ECLIA)
Device: Roche (Cobas C 501)
Department: Clinical Biochemistry
Specimen type: Serum
Specimen Volume: 1 mL
Turnaround time (TAT): Daily
Principle and method: Immunoassay
Device: Siemens (Immulite 2000 XPi)
Department: Allergy
Specimen type: Serum, Body Fluids, CSF
Specimen Volume: 1 mL
Special instructions: Samples of body fluids and CSF Must Taken By Specialized Physician then sent to the Lab.
Turnaround time (TAT): Daily
Principle and method: ElectroChemiLuminescence (ECLIA)
Device: Roche (Cobas C 501)
Department: Clinical Biochemistry
Specimen type: Serum
Specimen Volume: 1 mL
Turnaround time (TAT): Daily
Principle and method: ElectroChemiLuminescence (ECLIA)
Device: Roche (Cobas C 501)
Department: Clinical Biochemistry
Specimen type: Serum
Specimen Volume: 1 mL
Specimen stability: Sample is stable for 15 days at 2-8 C°.
Turnaround time (TAT): Daily
Principle and method: Kinetic
Department: Clinical Biochemistry
Specimen type: Serum, 24 Hour Urine
Specimen Volume: 1 mL (Serum)
Specimen stability: Samples are stable for 5 days at 2-8 C°, or 1 month at -20 C°.
Special instructions:
Serum: Note the time of collection and the position of patient (Supine, Upright).
24 Hour Urine Sample: See Urine Preservatives and 24 hour urine collection
Turnaround time (TAT): Daily (Serum), 2 days (24 Hour urine)
Principle and method: Chemiluminescence immunoassay (CLIA)
Device: Snibe (Maglumi)
Department: Endocrinology
Specimen type: Serum, Body Fluids (Pleural, Articular, Ascitic, Other body fluids)
Specimen Volume: 1 mL
Specimen stability: Sample is stable for 7 days at Room Temperature, 7 days at 4‑8 °C, 2 months at -20 °C.
Special instructions:
Body Fluid Sample Taken By Specialized Physician.
Limitations: Test Result slightly increases by hemolysis.
Turnaround time (TAT): Daily
Principle and method: ElectroChemiLuminescence (ECLIA)
Device: Roche (Cobas C 501)
Department: Clinical Biochemistry
Specimen: Serum
Principle and method: Nephlometry
Specimen Volume: 1 mL
Device: Siemens BN Prospec
Turnaround Time (TAT): Daily
Department: Clinical Biochemistry
Specimen type: Serum, Amniotic Fluid
Specimen Volume: 1 mL
Specimen stability: Sample is Stable for 5 days at 20‑25 °C, 14 days at 2‑8 °C, Or 6 months at ‑20 °C (± 5 °C). The samples may be frozen 3 times.
Limitations: The measured AFP value of a patient’s sample can vary depending on the testing procedure used. AFP values determined on patient samples by different testing procedures cannot be directly compared with one another and could be the cause of erroneous medical interpretations.
Turnaround time (TAT): Daily
Principle and method: electrochemiluminescence (ECLIA)
Device: Roche (Cobas e 601)
Department: Tumor Markers
Specimen: CSF, Lithium Heparin Plasma (Frozen), Urine
Principle and method: HPLC
Device: API 3200 LC/MS/MS System
Turnaround Time (TAT): 10 Days
Department: Advanced Clinical Chemistry
Specimen type: EDTA Plasma (Frozen)
Specimen Volume: 1 mL
Specimen stability: Sample is Stable for 3 weeks at -38 °C.
Special instructions: Collect blood from stasis‑free vein of fasting patient. Smoking should be avoided prior to sampling. Tubes should be filled completely and kept tightly stoppered at all times. Place immediately on ice and centrifuge, preferably at 4 °C. Perform analysis within 20‑30 minutes of venipuncture or freeze separated plasma immediately.
Limitations: Ammonia concentrations can increase in vitro due to breakdown of nitrogen‑containing plasma components. One known source of ammonia formation at storage higher than ‑38 °C is an increased
γ‑glutamyltransferase activity leading to decomposition of glutamine.
Avoid contamination of samples by ammonia from smoking or traffic in laboratory or patient’s room, glassware, or water.
Contamination with erythrocytes will elevate results, because the analyte level in erythrocytes is higher than in normal plasma. The level of interference may be variable depending on the content of analyte in the lysed erythrocytes.
Cefoxitin and Intralipid cause artificially high and low ammonia
results respectively at the therapeutic drug level.
Physiological plasma concentrations of Sulfasalazine and Sulfapyridine may lead to false results.
Temozolomide at therapeutic concentrations may lead to erroneous results.
Turnaround time (TAT): Daily
Principle and method: Enzymatic method.
Device: Roche (Cobas C 501)
Department: Clinical Biochemistry
Specimen: Chorio villi, Amniotic Fluid, Aborted Fetus
Principle: Karyotype
Turnaround Time (TAT): 1 Month
Department: Cytogenetics
Specimen: Serum, Urine, 24 Hour Urine, Body Fluid.
Specimen Stability: Serum: 7 days at 15‑25 °C, 1 month at 2‑8 °C. Urine: 2 days at 15‑25 °C, 10 days at 2‑8 °C.
Patient Preparation: See Instruction for 24 urine Collection
Special Instructions: Collect urine without additives. α‑Amylase is unstable in acid urine.
Assay promptly or adjust pH to alkaline range (just above pH 7) before storage.
Principle and method: Enzymatic colorimetric assay
Specimen Volume: 1 mL
Device: Roche Cobas C 501
Limitation: Highly turbid and grossly lipemic samples may cause Abs. flags.
Anticoagulants: Interference was found with citrate, fluoride, and EDTA.
Exception: Icodextrin‑based drugs may lead to decreased amylase results.
Turnaround Time (TAT): Daily
Department: Clinical Biochemistry
The ACCR is calculated from amylase activity and creatinine concentration.
Both the serum and urine samples should be collected at the same time.
Specimen: Serum, Urine.
Specimen Stability: Serum: 7 days at 15‑25 °C, 1 month at 2‑8 °C. Urine: 2 days at 15‑25 °C, 10 days at 2‑8 °C.
Special Instructions: Collect urine without additives. α‑Amylase is unstable in acid urine.
Assay promptly or adjust pH to alkaline range (just above pH 7) before storage.
Principle and method: Enzymatic colorimetric assay
Specimen Volume: 1 mL
Device: Roche Cobas C 501
Turnaround Time (TAT): Daily
Department: Clinical Biochemistry
Specimen: Serum.
Specimen Stability: Sample is stable for 24 hour at 2-8 C°, or for 2 months at -20 C°.
Principle and method: chemiluminescence enzyme immunoassay (CLIA)
Specimen Volume: 1 mL
Sample Rejection: Hemolysis, lipemic, ictric.
Device: Siemens Immulite 2000 XPi
Turnaround Time (TAT): Daily
Department: Endocrinology
Specimen: Serum, CSF
Principle and method: Photometric/Kinetics
Specimen Volume: 1 mL
Turnaround Time (TAT): Daily
Department: Clinical Biochemistry
Specimen: Serum
Specimen Stability: 3 days 2-8 C, Long storage require -20 C
Principle and method: ELISA
Specimen Volume: 1 mL
Turnaround Time (TAT): 1 Month
Department: Immunology & Serology
Specimen: Serum.
Specimen Stability: 4 days at 2/8°C, or frozen for longer periods at –20°C
Principle and method: ELISA
Specimen Volume: 1 mL
Device: Diesse Chorus Trio
Turnaround Time (TAT): 2 Days
Department: Immunology & Serology
Specimen: Serum.
Specimen Stability: 4 days at 2/8°C, or frozen for longer periods at –20°C
Principle and method: ELISA
Specimen Volume: 1 mL
Device: Diesse Chorus Trio
Turnaround Time (TAT): 2 Days
Department: Immunology & Serology
Specimen: Serum.
Specimen Stability: 4 days at 2/8°C, or frozen for longer periods at –20°C
Principle and method: ELISA
Specimen Volume: 1 mL
Device: Diesse Chorus Trio
Turnaround Time (TAT): 2 Days
Department: Immunology & Serology
Specimen: Serum.
Specimen Stability: 4 days at 2/8°C, or frozen for longer periods at –20°C
Principle and method: ELISA
Specimen Volume: 1 mL
Device: Diesse Chorus Trio
Turnaround Time (TAT): 2 Days
Department: Immunology & Serology
Specimen: Serum.
Specimen Stability: 4 days at 2/8°C, or frozen for longer periods at –20°C
Principle and method: ELISA
Specimen Volume: 1 mL
Device: Diesse Chorus Trio
Turnaround Time (TAT): 2 Days
Department: Immunology & Serology
Specimen: Serum.
Specimen Stability: 3 days at 2‑8 °C, 1 month at ‑20 °C
Principle and method: ECLIA
Specimen Volume: 1 mL
Device: Roche E170
Turnaround Time (TAT): Daily
Department: Immunology & Serology
Limitations: The anti‑CCP test results can be false negative in patients with hypergammaglobulinemia. Results from patients suffering from this disorder should not be used for diagnostic purposes.
Specimen: Serum.
Specimen Stability: 4 days at 2/8°C, or frozen for longer periods at –20°C
Principle and method: ELISA
Specimen Volume: 1 mL
Device: Diesse Chorus Trio
Turnaround Time (TAT): 2 Days
Department: Immunology & Serology
Specimen: Serum.
Specimen Stability: 4 days at 2/8°C, or frozen for longer periods at –20°C
Principle and method: ELISA
Specimen Volume: 1 mL
Device: Diesse Chorus Trio
Turnaround Time (TAT): 2 Days
Department: Immunology & Serology
Specimen: Serum.
Specimen Stability: Up to 48 h at 2-8°C, or frozen at -20°C for longer periods
Principle and method: immunofluorescence (IF)
Specimen Volume: 1 mL
Turnaround Time (TAT): Weekly
Department: Immunology & Serology
Specimen: Serum.
Specimen Stability: Up to 48 h at 2-8°C, or frozen at -20°C for longer periods
Principle and method: immunofluorescence (IF)
Specimen Volume: 1 mL
Turnaround Time (TAT): Weekly
Department: Immunology & Serology
Specimen: Serum.
Specimen Stability: 4 days at 2-8°C, or frozen at -20°C for longer periods
Principle and method: ELISA
Device: Diesse Chorus Trio
Specimen Volume: 1 mL
Turnaround Time (TAT): 3 Days
Department: Immunology & Serology
Specimen: Serum.
Specimen Stability: Up to 48h at 2-8°C, or frozen at -20°C for longer periods.
Principle and method: ELISA
Specimen Volume: 1 mL
Turnaround Time (TAT): 3 Days
Department: Immunology & Serology
Specimen: Serum.
Specimen Stability: 4 days at 2/8°C, or frozen
for longer periods at –20°C.
Device: Diesse Chorus Trio
Principle and method: ELISA
Specimen Volume: 1 mL
Turnaround Time (TAT): Weekly
Department: Immunology & Serology
Specimen: Serum.
Specimen Stability: 4 days at 2/8°C, or frozen
for longer periods at –20°C.
Device: Diesse Chorus Trio
Principle and method: ELISA
Specimen Volume: 1 mL
Turnaround Time (TAT): Weekly
Department: Immunology & Serology
Specimen: Serum.
Specimen Stability: 4 days at 2/8°C, or frozen
for longer periods at –20°C.
Device: Diesse Chorus Trio
Principle and method: ELISA
Specimen Volume: 1 mL
Turnaround Time (TAT): Daily
Department: Immunology & Serology
Specimen: Serum.
Specimen Stability: 5 days at 2/8°C, or frozen
for longer periods at –20°C.
Device: Snibe Maglumi 2000 Plus
Principle and method: CLIA
Specimen Volume: 1 mL
Turnaround Time (TAT): Daily
Department: Immunology & Serology
Specimen: Serum.
Specimen Stability: 4 days at 2/8°C, or frozen
for longer periods at –20°C.
Device: Diesse Chorus Trio
Principle and method: ELISA
Specimen Volume: 1 mL
Turnaround Time (TAT): Daily
Department: Immunology & Serology
